Part:BBa_K4397004

Amyloid-Beta - 7-92-1H1

Amyloid-Beta - 7-92-1H1

Target: Aβ(42) monomer

Binding Affinity: 63.4 nM (SPR)

Usage and Biology

Aβ7-92-1H1 is a DNA aptamer with a length of 44 bases containing a stem-loop structure. Aβ7-92-1H1 Kd value of 63.4 nM indicated high specificity of this aptamer for Aβ42 monomer [1]. It has been shown that Aβ7-92-1H1 could not bind to Aβ40 monomer, but it recognizes Aβ42 and Aβ40 aggregates (oligomer and fibril) with various affinities [1]. This indicates that the selectivity of Aβ7-92-1H1 for Aβ40 monomer needs to be improved. Still, its ability to recognize Aβ monomers and aggregates is very suitable for the development of inhibitors for regulating Aβ aggregation [1].

[1] Zheng, Y., Zhang, L., Zhao, J., Li, L., Wang, M., Gao, P., ... & Wang, W. (2022). Advances in aptamers against Aβ and applications in Aβ detection and regulation for Alzheimer's disease. Theranostics, 12(5), 2095.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]